Fig. 1

Expression of B7-H4 on human and mice dDC subsets infected with T. gondii. a Flow cytometry analyses of B7-H4 levels in mouse CD11c⁺CD8a⁻ and CD11c⁺CD8a⁺ dDCs from WT uninfected, WT infected, and B7-H4^−/− pregnant female mice. b Histogram analysis of B7-H4 expression changes in CD11c⁺CD8a⁻ and CD11c⁺CD8a⁺ dDCs from WT uninfected, WT infected, or B7-H4^−/− infected groups of mice. c Flow cytometry analyses of B7-H4 levels on human decidual MDC1, MDC2, and PDC subsets from uninfected, infected, and B7-H4-neutralized infected groups. d Histogram analysis of B7-H4 expression changes in human decidual MDC1, MDC2, and PDC subsets from uninfected, infected, and B7-H4-neutralized and infected groups. e Representative images of IF of B7-H4 (green) expression levels in purified human dDCs from uninfected, infected, or B7-H4-neutralized infected groups. DAPI (blue) labeling defined the morphology of nuclei. Scale bars, 10 μm. f Histogram analysis of B7-H4 expression changes on purified human dDCs by immunofluorescence intensity among three groups. g Western blot analysis of B7-H4 levels in purified human dDCs from uninfected, infected, and B7-H4-neutralized infected groups. IFI, immunofluorescence intensity. Data shown are means ± SD. At least six samples of human decidua in each group were assayed individually in flow cytometry and at least three samples of human decidua in each group were assayed individually in Western blot and IF by paired t-test for uninfected vs infected group, infected vs B7-H4-neutralized group. At least eight pregnant female mice in each group were assayed individually by unpaired t-test for uninfected vs infected group, infected vs B7-H4^−/−infected group