Fig. 2
Real-time PCR amplification and calibration curves of the three primer and probe sets used in the detection of Babesia parasites in the current study. a Amplification plots. b Standard curves. The Babesia bovis 18S primer set had an efficiency of 98.96%, a slope of –3.34 and a correlation coefficient (R2) of 0.981, while the cytochrome b primers had an efficiency of 90.4%, a slope − 3.57 and a R2 of 0.999. The B. bigemina set had an efficiency of 99.51%, a slope of − 3.33 and a R2 of 1.0. The plots and standard curves were generated using QuantStudio design and analysis software version 2.6.0. The detection limit of the gene in the control DNA was 10−7 dilution for B. bovis 18S primers, 10−6 for cytochrome b and 10−4 for B. bigemina cytochrome b