Table 2 High-confidence tick proteins with reported functions in the hemolymph of H. flava ticks
From: Protein profiling of hemolymph in Haemaphysalis flava ticks
No. | Protein | Alignment | iBAQ (× 106) | ||||
|---|---|---|---|---|---|---|---|
ID | Length (amino acids) | Entry and overview | E value | Score | Identity (%) | ||
I. Enzymes | |||||||
1 | Cl-k.18156 ①| 376 | L7M876, tick serine protease, Rhipicephalus pulchellus | 0 | 1897 | 84.6 | 5.96 ± 1.32 |
2 | Cl-k.17502 ①| 484 | A0A131Z7A0, tick serine protease, Rhipicephalus appendiculatus | 0 | 1451 | 67.8 | 0.39 ± 0.04 |
3 | Cl-k.19217 ①| 437 | A0A1E1X8K7, serine protease, Amblyomma aureolatum | 0 | 1907 | 85.1 | 2.13 ± 0.47 |
4 | Cl-k.19341 | 348 | A1IHG0, longipain, H. longicornis | 0 | 1814 | 93.5 | 7.34 ± 1.74 |
5 | Cl-k.18108 | 443 | A0A097CK68, enolase, H. flava | 0 | 2233 | 99.5 | 1.16 ± 0.35 |
6 | Cl-k.14381 | 507 | A0A6M2D6D9, serine carboxypeptidase, R. microplus | 0 | 2115 | 83.6 | 0.49 ± 0.15 |
7 | Cl-k.18093 | 164 | A0A131XJQ8, metalloproteinase, Hyalomma excavatum | 1.7E−91 | 686 | 75.0 | (7) 221.26 ± 58.79 |
8 | Cl-k.18993 | 398 | Q2WFX6, aspartic protease, H. longicornis | 0 | 1990 | 95.9 | 1.47 ± 0.34 |
9 | Cl-k.7217 | 397 | A0A034WWI5, heme-binding aspartic peptidase, R. microplus | 0 | 1343 | 68.0 | 3.03 ± 0.93 |
10 | Cl-k.14313 | 561 | A0A1E1XAU4, cysteine proteinase, A. aureolatum | 0 | 2602 | 84.4 | 5.24 ± 1.17 |
11 | Cl-k.18480 | 326 | A0A023FWK4, cathepsin L, Amblyomma parvum | 0 | 1604 | 88.7 | 99.93 ± 26.58 |
12 | Cl-k.24797 | 110 | A0A023GJU1, cathepsin C, Amblyomma triste | 2.6E−70 | 563 | 92.7 | 2.08 ± 0.83 |
13 | Cl-k.18626 | 416 | L7M0J1, phospholipase a2, R. pulchellus | 0 | 1704 | 80.1 | 25.19 ± 5.41 |
14 | Cl-k.30316 ② | 566 | Q9U6M8, carboxylic ester hydrolase, R. microplus | 5.1E−171 | 1290 | 47.3 | 10.59 ± 2.67 |
15 | Cl-k.24717 ② | 564 | A0A6M2CHI4, carboxylic ester hydrolase, R. microplus | 0 | 1366 | 49.7 | 0.25 ± 0.05 |
16 | Cl-k.18635 | 521 | A5LHV9, disulfide isomerase, H. longicornis | 0 | 2514 | 96.2 | 1.74 ± 0.43 |
17 | Cl-k.17461 | 163 | F2Z7L0, lysozyme, H. longicornis | 3.1E−70 | 544 | 87.1 | 2.44 ± 0.86 |
18 | Cl-k.17638 | 397 | G8C7A0, lysosomal acid phosphatase, H. longicornis | 0 | 1878 | 93.5 | 75.50 ± 18.32 |
19 | Cl-k.18136 | 233 | Q6JVN0, glutathione S-transferase, H. longicornis | 3.5E−159 | 1144 | 93.7 | 1.74 ± 0.50 |
20 | Cl-k.18835 | 526 | A0A131YK94, superoxide dismutase (Cu–Zn), R. appendiculatus | 0 | 1475 | 61.8 | 18.83 ± 4.63 |
21 | Cl-k.19315 ③ | 316 | A0A131YMH9, chitinase, R. appendiculatus | 0 | 1777 | 85.6 | 1.46 ± 0.44 |
22 | Cl-k.18448 ③ | 536 | A0A023FND8, chitinase, A. cajennense | 0 | 1919 | 80.3 | 21.40 ± 4.31 |
23 | Cl-k.17863 | 367 | A0A286R6W4, fructose-bisphosphate aldolase, Dermacentor silvarum | 0 | 1796 | 94.2 | 2.26 ± 0.57 |
24 | Cl-k.35642 | 331 | A0A1E1X9Y4, alpha-L-fucosidase, A. aureolatum | 0 | 1414 | 81.7 | 1.87 ± 0.38 |
25 | Cl-k.18180 | 430 | A0A2P1DPZ4, glyceraldehyde-3-phosphate dehydrogenase, H. flava | 0 | 1730 | 100 | 2.37 ± 0.62 |
26 | Cl-k.17933 | 549 | A0A6M2CTD4, ATP synthase subunit beta, R. microplus | 0 | 2628 | 96.3 | 0.39 ± 0.02 |
II. Enzyme inhibitors | |||||||
27 | Cl-k.20245 ④ | 256 | A0A5P8H6S1, serpin-a, H. longicornis | 9.3E−97 | 753 | 61.9 | 55.88 ± 13.09 |
28 | Cl-k.17714 ④ | 415 | A0A5P8H6S1, serpin-a, H. longicornis | 6.9E−169 | 1246 | 63.4 | 0.25 ± 0.24 |
29 | Cl-k.16905 ④ | 143 | A0A5P8H6S1, serpin-a, H. longicornis | 6.4E−49 | 422 | 61.8 | 32.01 ± 6.67 |
30 | Cl-k.18212 ④ | 427 | A0A5P8H6S1, serpin-a, H. longicornis | 0 | 1727 | 87.5 | 45.04 ± 10.59 |
31 | Cl-k.19946 ④ | 406 | Q75Q63, serpin-2 , H. longicornis | 0 | 1686 | 82.4 | 11.48 ± 2.66 |
32 | Cl-k.16646 ④ | 201 | A0A6M2E637, tick serpins 8, A. tuberculatum | 7.5E−68 | 555 | 63.0 | ⑻ 178.59 ± 44.58 |
33 | Cl-k.22217 ④ | 398 | A0A023GN51, tick serpins 13, A. triste | 9.5E−165 | 1216 | 61.8 | 38.49 ± 10.90 |
34 | Cl-k.14429 | 421 | A0A023G8Z1, serine proteinase inhibitor, A. triste | 0 | 1409 | 65.5 | 71.47 ± 17.26 |
35 | Cl-k.18644 ⑤ | 229 | A0A224YJB7, α2-macroglobulin splicing variant, Rhipicephalus zambeziensis | 3.7E−141 | 1119 | 91.7 | ⑸ 435.08 ± 100.49 |
36 | Cl-k.18677 ⑤ | 1142 | A0A1E1XEL3, α-macroglobulin, A. aureolatum | 0 | 5401 | 89.6 | ⑹ 243.02 ± 53.71 |
37 | Cl-k.19779 ⑤ | 1820 | A0A1E1XL07, α-macroglobulin, A. sculptum | 0 | 6650 | 76.1 | 1.10 ± 0.27 |
38 | Cl-k.18726 et al. | 1915 | A0A023FNM2, α2-macroglobulin splicing variant, Amblyomma cajennense | 0 | 5819 | 78.9 | 35.72 ± 8.99 |
39 | Cl-k.18944 | 269 | A0A023GP16, Kazal-type serine protease inhibitor, A. triste | 5.8E−155 | 1123 | 84.0 | 8.02 ± 1.79 |
40 | Cl-k.12087 ⑥ | 183 | A0A6B9DA14, cystatin, H. flava | 0 | 757 | 100 | 15.11 ± 4.89 |
41 | Cl-k.17388 ⑥ | 185 | A0A3G6VF56, cystatin, H. flava | 1.9E−100 | 745 | 99.3 | 65.43 ± 15.54 |
42 | Cl-k.20981 ⑥ | 164 | A0A6M3YRY3, cystatin, H. flava | 4.2E−89 | 667 | 100 | 15.73 ± 4.93 |
43 | Cl-k.21288 | 228 | A0A023GEH6, thyropin, A. triste | 1.5E−94 | 723 | 60.0 | 22.19 ± 7.67 |
44 | Cl-k.23450 | 229 | A0A023GAB0, thyropin, A. triste | 2.2E−89 | 689 | 59.4 | 35.86 ± 8.77 |
III. Immune-related proteins | |||||||
45 | Cl-k.18200 ⑦ | 84 | A0A6G5A751, microplusin, R. microplus | 9.5E−31 | 260 | 54.8 | ⑴ 6514.56 ± 803.43 |
46 | Cl-k.18906 ⑦ | 134 | A0A6G5A751, microplusin, R. microplus | 8.0E−34 | 295 | 52.5 | ⑷ 699.92 ± 23.27 |
47 | Cl-k.20235 ⑦ | 87 | A0A6G5A751, microplusin, R. microplus | 2.1E−29 | 266 | 46.2 | 61.60 ± 17.16 |
48 | Cl-k.3924 | 78 | A0A2D1CLH7, defensin DFS2, H. longicornis | 2.1E−48 | 361 | 83.6 | 11.98 ± 3.53 |
49 | Cl-k.23590 ⑧ | 1612 | A0A131ZDX3, TIL domain-containing protein, R. appendiculatus | 0 | 7053 | 77.0 | 11.48 ± 2.56 |
50 | Cl-k.13586 ⑧ | 2610 | A0A131Z678, TIL domain-containing protein, R. appendiculatus | 0 | 8535 | 77.2 | 9.72 ± 2.21 |
51 | Cl-k.18775 ⑧ | 2252 | A0A131YJS1, TIL domain-containing protein, R. appendiculatus | 0 | 8966 | 68.9 | 1.42 ± 0.30 |
52 | Cl-k.25067 ⑧ | 109 | A0A6M2CNI0,TIL domain-containing protein, R. microplus | 4.1E−39 | 329 | 69.9 | 26.91 ± 4.60 |
53 | Cl-k.18521 ⑨ | 235 | F0J8I6, ixoderin, A. variegatum | 5.4E−93 | 707 | 66.3 | 6.23 ± 1.40 |
54 | Cl-k.17959 ⑨ | 313 | A0A1E1XEF5, ixoderin, A. aureolatum | 5.7E−153 | 1118 | 73.8 | 6.92 ± 1.81 |
55 | Cl-k.19166 | 177 | A0A6M2D1K3, C2b, R. microplus | 6.9E−55 | 488 | 62.3 | 2.75 ± 0.88 |
56 | Cl-k.21838 | 428 | A0A7L9DI94, C3, Ixodes ricinus | 1.0E−134 | 1104 | 50.9 | 10.48 ± 2.55 |
57 | Cl-k.14141 | 152 | A0A224Z7V2, serum amyloid A protein, R. zambeziensis | 1.4E−77 | 592 | 72.7 | 28.19 ± 6.81 |
58 | Cl-k.17842 | 332 | A0A0S3Q1T5, leucine-rich repeat containing protein, H. longicornis | 0 | 1464 | 90.1 | 19.96 ± 4.17 |
59 | Cl-k.18342 | 233 | A0A0M3TC17, AV422, H. flava | 8.4E−171 | 1221 | 100 | 12.15 ± 2.84 |
60 | Cl-k.18520 ⑩ | 181 | Q08G07, Hq05, Haemaphysalis qinghaiensis | 1.1E−129 | 940 | 97.2 | 6.53 ± 1.43 |
61 | Cl-k.18575 ⑩ | 180 | G3BJU6, immunogenic protein, H. longicornis | 4.5E−120 | 877 | 93.9 | 16.62 ± 4.03 |
IV. Transporters | |||||||
62 | Cl-k.25224 ⑪ | 1538 | Q5EG54, Vg, D. variabilis | 0 | 5360 | 67.5 | 0.21 ± 0.10 |
63 | Cl-k.16576 ⑪ | 686 | E1CAX9, vitellogenin-1, H. longicornis | 0 | 1248 | 98.5 | 126.50 ± 31.13 |
64 | Cl-k.19213-k18886 ⑪ | 351 | B1B544, vitellogenin-2, H. longicornis | 8.7E−86 | 732 | 98.3 | ⑶ 1606.10 ± 295.28 |
65 | Cl-k.18067 ⑪ | 463 | B1B544, vitellogenin-2, H. longicornis | 0 | 2072 | 85.1 | ⑵ 1829.13 ± 312.65 |
66 | Cl-k.19483 ⑪ | 1386 | B1B544, vitellogenin-2, H. longicornis | 0 | 4657 | 62.4 | ⑽ 138.31 ± 29.98 |
67 | Cl-k.16789 ⑪ | 114 | E1CAY0, vitellogenin-3, H. longicornis | 3.1E−62 | 535 | 85.7 | 95.40 ± 25.38 |
68 | Cl-k.18851-18114 ⑪ | 2279 | G9M4L6, vitellogenin B, H. longicornis | 0 | 13,028 | 85.9 | 63.60 ± 12.52 |
69 | Cl-k.21299 | 207 | M5AYG7, ferritin 2, H. longicornis | 9.9E−133 | 965 | 93.5 | 22.51 ± 4.62 |
70 | Cl-k.19103 | 152 | A0A023G718, fatty acid-binding protein, A. triste | 4.6E−83 | 628 | 85.4 | 15.26 ± 2.76 |
V. Muscle proteins | |||||||
71 | Cl-k.18720 | 877 | J7LVN2, paramyosin, H. longicornis | 0 | 4196 | 98.2 | 0.53 ± 0.13 |
72 | Cl-k.18460 | 424 | A8E4J9, calreticulin, H. qinghaiensis | 0 | 2247 | 99.5 | 2.42 ± 0.76 |
73 | Cl-k.18394 | 52 | A0A131ZAE8, tropomyosin, R. appendiculatus | 0 | 1423 | 93.2 | 3.76 ± 1.07 |
74 | Cl-k.18452 | 83 | A0A0N6X2B1, muscle LIM protein, H. longicornis | 7.8E−57 | 417 | 95.8 | 4.98 ± 1.14 |
VI. Heat shock proteins | |||||||
75 | Cl-k.18505 ⑫ | 655 | A0A097A1J8, heat shock 70 kDa protein 8, H. flava | 0 | 3290 | 100 | 2.09 ± 0.55 |
76 | Cl-k.18161 ⑫ | 683 | E4W3Z2, heat shock 70 kDa protein 5, H. longicornis | 0 | 3317 | 99.1 | 0.45 ± 0.22 |
Other | |||||||
Cl-k.18334 | 255 | A0A023FPM9, glycine-rich secreted cement protein, A. cajennense | 1.1E−118 | 884 | 74.0 | ⑼ 170.44 ± 38.51 | |