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Table 2 Real Time PCR results for the spleens and ears of the 10 Leishmania infantum-positive Rattus norvegicus captured in the Barcelona sewer system

From: Molecular detection of Leishmania infantum in rats and sand flies in the urban sewers of Barcelona, Spain

Rattus norvegicus ID numbers qPCR results a
R. norvegicus spleens Ct Estimated n of parasites/100 mg Standard deviation
1 35.45 6.71 2.34
2 35.24 8.43 2.22
3 28.06 794.89 74.66
4 35.24 8.43 2.22
5 35.07 29.43 3.49
6 23.10 20,156.21 1,987.96
7 30.06 445.04 38.93
R. norvegicus ears Ct Estimated n of parasites/150 mg tissue Standard deviation
1 29.82 1222.35 242.37
8 18.09 430,119.63 86,890
9 34.87 10.66 2.32
10 33.86 84.33 37.63
  1. Cut-off established for a positive result: Ct values < 36
  2. a Mean values of the three replicates of each sample