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Table 2 Real Time PCR results for the spleens and ears of the 10 Leishmania infantum-positive Rattus norvegicus captured in the Barcelona sewer system

From: Molecular detection of Leishmania infantum in rats and sand flies in the urban sewers of Barcelona, Spain

Rattus norvegicus ID numbers

qPCR results a

R. norvegicus spleens

Ct

Estimated n of parasites/100 mg

Standard deviation

1

35.45

6.71

2.34

2

35.24

8.43

2.22

3

28.06

794.89

74.66

4

35.24

8.43

2.22

5

35.07

29.43

3.49

6

23.10

20,156.21

1,987.96

7

30.06

445.04

38.93

R. norvegicus ears

Ct

Estimated n of parasites/150 mg tissue

Standard deviation

1

29.82

1222.35

242.37

8

18.09

430,119.63

86,890

9

34.87

10.66

2.32

10

33.86

84.33

37.63

  1. Cut-off established for a positive result: Ct values < 36
  2. a Mean values of the three replicates of each sample