Fig. 1

AgDNV infects the reproductive tract of Anopheles gambiae males. a–c Males were injected with either AgDNV (DNV on figure) or PBS (control). a–c At 11, 13, 15 and 18 dpi, AgDNV-treated males have infected: a testes [Ln(y) transformed, one-way ANOVA, Sidak’s correction], b MAGs [Ln(y) transformed, one-way ANOVA, Sidak’s correction], c carcass [Ln(y) transformed, Brown-Forsythe/Welch ANOVA, Dunnett’s correction]. However, viral titers do not change over time in any tissue. d Independent of time (including 11-18 dpi), AgDNV titers in the MAGs and carcass are significantly higher than those in the testes [Ln(y) transformed, Brown-Forsythe/Welch ANOVA, Dunnett’s correction]. NS indicates P-value > 0.05. Throughout, for testes and MAGs, each point represents a pool of 12 tissues; for carcasses, each point represents an individual carcass with the reproductive tract removed. Viral genomes are normalized to host genomes (S7). Abbreviations: AgDNV, Anopheles gambiae densovirus; ANOVA, analysis of variance; dpi, days post-injection; Ln(y), log transformed; MAGs, male accessory glands; PBS, phosphate-buffered saline; S7, host ribosomal protein gene; VP, viral protein