Table 2 Wolbachia density in wMel and wAlbB Ae. aegypti (Pud) release lines on exposure in larval stage to temperatures maintained at constantly and different ranges of diurnal cyclic temperatures in laboratory and under sunlight (natural) compared to the respective line exposed to a constant temperature of 26 ± 1 °C (control) in laboratory

wMel male

 26 °C to 40 °C*

8

200

1.33 (0.92)

0.39–2.41

8

200

9.17 (7.67)

2.05–19.14

 26 °C to 36 °C*

8

200

2.20 (0.94)

1.14–3.91

8

200

3.07 (0.56)

2.42–3.93

 26 °C to 30 °C

8

200

3.99 (0.89)

2.44–4.85

8

200

3.85 (1.82)

2.07–6.44

 30 °C*

8

200

1.56 (0.39)

1.08–2.18

8

200

2.73 (0.77)

1.57–3.81

 Full open bowls*

3

150

2.05 (0.54)

1.66–2.66

4

100

16.02 (3.47)

11.38–19.14

 Half open bowls*

3

150

0.75 (0.24)

0.47–0.93

4

100

16.02 (3.47)

11.38–19.14

wMel female

 26 °C to 40 °C

8

200

7.36 (4.48)

1.50–12.17

8

200

8.29 (3.67)

4.57–13.75

 26 °C to 36 °C

8

200

6.20 (3.90)

2.55–11.76

8

200

7.29 (4.28)

3.19–12.44

 26 °C to 30 °C

8

200

11.44 (5.40)

1.35–16.70

8

200

10.67 (4.08)

4.63–15.90

 30 °C

8

200

10.16 (3.91)

5.59–17.05

8

200

11.61 (3.76)

7.54–17.75

 Full open bowls*

3

150

10.91 (0.93)

9.89–11.72

4

100

5.05 (0.55)

4.57–5.80

 Half open bowls

3

150

5.57 (4.16)

3.05–10.37

4

100

5.05 (0.55)

4.57–5.80

wAlbB male

 26 °C to 40 °C*

8

200

31.68 (6.03)

24.29–38.68

8

200

37.35 (4.21)

28.47–41.15

 26 °C to 36 °C

8

200

29.67 (7.27)

17.39–36.23

8

200

23.96 (11.09)

13.65–40.43

 26 °C to 30 °C

8

200

18.10 (14.61)

3.61–33.44

8

200

29.63 (2.88)

25.53–33.80

 30 °C

8

200

28.95 (5.28)

22.43–39.86

8

200

30.00 (7.51)

20.69–38.51

 Full open bowls

3

150

32.99 (4.81)

28.23–37.85

4

100

35.82 (5.70)

28.47–40.39

 Half open bowls*

3

150

23.46 (0.91)

22.77–24.50

4

100

35.82 (5.70)

28.47–40.39

wAlbB female

 26 °C to 40 °C

8

200

24.09 (11.63)

12.23–39.69

8

200

31.06 (8.11)

20.87–43.07

 26 °C to 36 °C

8

200

24.25 (10.01)

10.90–43.56

8

200

17.77 (11.14)

9.07–41.56

 26 °C to 30 °C*

8

200

19.35 (4.46)

14.77–27.91

8

200

23.61 (2.93)

20.03–28.65

 30 °C

8

200

22.99 (6.78)

15.68–34.64

8

200

29.52 (6.90)

16.39–39.46

 Full open bowls

3

150

34.53 (5.15)

29.33–39.63

4

100

26.22 (5.19)

20.87–32.67

 Half open bowls

3

150

23.65 (4.80)

20.25–29.15

4

100

26.22 (5.19)

20.87–32.67