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Table 2 Wolbachia density in wMel and wAlbB Ae. aegypti (Pud) release lines on exposure in larval stage to temperatures maintained at constantly and different ranges of diurnal cyclic temperatures in laboratory and under sunlight (natural) compared to the respective line exposed to a constant temperature of 26 ± 1 °C (control) in laboratory

From: Sensitivity of wMel and wAlbB Wolbachia infections in Aedes aegypti Puducherry (Indian) strains to heat stress during larval development

Temperature/condition Experiment Control (26 ± 1°C constant)
Replicate n# Mean (SD) Min.–max. Replicate n# Mean (SD) Min.–max.
wMel male
 26 °C to 40 °C* 8 200 1.33 (0.92) 0.39–2.41 8 200 9.17 (7.67) 2.05–19.14
 26 °C to 36 °C* 8 200 2.20 (0.94) 1.14–3.91 8 200 3.07 (0.56) 2.42–3.93
 26 °C to 30 °C 8 200 3.99 (0.89) 2.44–4.85 8 200 3.85 (1.82) 2.07–6.44
 30 °C* 8 200 1.56 (0.39) 1.08–2.18 8 200 2.73 (0.77) 1.57–3.81
 Full open bowls* 3 150 2.05 (0.54) 1.66–2.66 4 100 16.02 (3.47) 11.38–19.14
 Half open bowls* 3 150 0.75 (0.24) 0.47–0.93 4 100 16.02 (3.47) 11.38–19.14
wMel female
 26 °C to 40 °C 8 200 7.36 (4.48) 1.50–12.17 8 200 8.29 (3.67) 4.57–13.75
 26 °C to 36 °C 8 200 6.20 (3.90) 2.55–11.76 8 200 7.29 (4.28) 3.19–12.44
 26 °C to 30 °C 8 200 11.44 (5.40) 1.35–16.70 8 200 10.67 (4.08) 4.63–15.90
 30 °C 8 200 10.16 (3.91) 5.59–17.05 8 200 11.61 (3.76) 7.54–17.75
 Full open bowls* 3 150 10.91 (0.93) 9.89–11.72 4 100 5.05 (0.55) 4.57–5.80
 Half open bowls 3 150 5.57 (4.16) 3.05–10.37 4 100 5.05 (0.55) 4.57–5.80
wAlbB male
 26 °C to 40 °C* 8 200 31.68 (6.03) 24.29–38.68 8 200 37.35 (4.21) 28.47–41.15
 26 °C to 36 °C 8 200 29.67 (7.27) 17.39–36.23 8 200 23.96 (11.09) 13.65–40.43
 26 °C to 30 °C 8 200 18.10 (14.61) 3.61–33.44 8 200 29.63 (2.88) 25.53–33.80
 30 °C 8 200 28.95 (5.28) 22.43–39.86 8 200 30.00 (7.51) 20.69–38.51
 Full open bowls 3 150 32.99 (4.81) 28.23–37.85 4 100 35.82 (5.70) 28.47–40.39
 Half open bowls* 3 150 23.46 (0.91) 22.77–24.50 4 100 35.82 (5.70) 28.47–40.39
wAlbB female
 26 °C to 40 °C 8 200 24.09 (11.63) 12.23–39.69 8 200 31.06 (8.11) 20.87–43.07
 26 °C to 36 °C 8 200 24.25 (10.01) 10.90–43.56 8 200 17.77 (11.14) 9.07–41.56
 26 °C to 30 °C* 8 200 19.35 (4.46) 14.77–27.91 8 200 23.61 (2.93) 20.03–28.65
 30 °C 8 200 22.99 (6.78) 15.68–34.64 8 200 29.52 (6.90) 16.39–39.46
 Full open bowls 3 150 34.53 (5.15) 29.33–39.63 4 100 26.22 (5.19) 20.87–32.67
 Half open bowls 3 150 23.65 (4.80) 20.25–29.15 4 100 26.22 (5.19) 20.87–32.67
  1. *Statistically significant
  2. #Number of larvae exposed