Table 1 Primers sequences used in the current study
Target organism | Primer sequence | Amplified gene/ length (bp) | Reference |
|---|---|---|---|
Babesia spp. | 5’-GTCTTGTAATTGGAATGATGG-3’ 5’-CCAAAGACTTTGATTTCTCTC-3’ | ssu-rRNA/560 | [12] |
Hepatozoon canis | 5’-ATACATGAGCAAAATCTCAAC-3’ 5’- CTTATTATTCCATGCTGCAG-3’ | 18S-rRNA/666 | [19] |
Anaplasmataceae | 5’-TTTATCGCTATTAGATGAGCCTATG-3’ 5’-CTCTACACTAGGAATTCCGCTAT -3’ | 16S-rRNA/450 | [20] |