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Fig. 4 | Parasites & Vectors

Fig. 4

From: Infection susceptibility and vector competence of Rhodnius robustus Larrousse, 1927 and R. pictipes Stal, 1872 (Hemiptera, Reduviidae, Triatominae) for strains of Trypanosoma cruzi (Chagas, 1909) (Kinetoplastida, Trypanosomatidae) I, II and IV

Fig. 4

Silver staining of products in 6% polyacrylamide gel electrophoresis show the 24Sα rDNA gene of T. cruzi strains (TcIAM, TcIMG, TcIIPR and TcIVAM) in the intestinal contents of R. robustus and R. pictipes with pure and mixed infections. Lanes (from left to right): 1, 100-bp molecular weight (MW) standard; 2, negative control (NC); 3–8, standard DNA of the 6 T. cruzi DTUs (TcI–TcVI); 9–11, 18, 20 and 22, negative samples for T. cruzi; 12, TcIAM+TcIVAM (G5); 14, TcIMG+TcIVAM (G7); 15, TcIIPR+TcIVAM (G9); 16, TcIAM+TcIIPR (G6); 17, TcIMG+TcIIPR (G8); 19, TcIAM (G1); 21, TcIMG (G2); 23, TcIIPR (G3); 24, 25, TcIAM+TcIIPR (G6); 26, 27, TcIMG+TcIIPR (G8); 28, TcIIPR+TcIVAM (G9); 29, negative control for PCR (NCPCR; no DNA). Abbreviations: DTUs, discrete typing units

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