Fig. 1

a, b Differentially expressed (DE) transcripts in Rhodnius prolixus fat body in response to injections of Gram-negative (Gr-) and Gram-positive (Gr+) bacteria. a Venn diagrams of DE transcripts. Numbers within the circles represent the number of DE transcripts with a false discovery rate P-value < 0.05; triangles indicate upregulated transcripts, inverted triangles indicate downregulated transcripts. Comparisons of bacteria at 8 (Venn diagram top) and 24 h post-infection (hpi) (Venn diagram second from top) use the respective phosphate-buffered saline (PBS) time point control as a reference to calculate DE. Comparisons of DE through time (Venn diagram third from top) used 8-hpi time points as a reference for DE estimations. Comparisons between bacterial treatments used the Gr+ bacteria as the reference. b Distribution and counts of biological process gene ontology (GO) terms in DE transcripts. Bars are colored according to the respective group in the Venn diagrams, blue for Gr− bacteria, red for Gr+ bacteria, black for PBS, white for comparisons between bacteria 8 hpi, and purple for comparisons between bacteria 24 hpi. Bars above the x-axis represent upregulated genes, bars below the x-axis represent the number of downregulated genes. Individual transcripts can have multiple GO terms. We display biological process GO terms at level 2 in this figure