Enhanced procedures for mosquito identification by MALDI-TOF MS

Parasites & Vectors

Table 1 Overview of mosquito origins, subgroups for database creation and parameters assessed

Species	Strains^a	Country	Source	Stored	Number (engorged)^b	DB1^c	DB2^c	DB3^c	Parameters assessed^d
Aedes aegypti	Bora Bora (Bora)	French Polynesia	Laboratory	Fresh	155 (110)	6^e	6^e	6^e	A, B, C
Ae. aegypti	Oiapoque (Oia)	Brazil	Field	Frozen (- 20 °C)	15			2^f	C
Ae. albopictus	Marseille (Mrs)	France	Laboratory	Fresh	15		2^f	2^f	C
Ae. albopictus	Dschang (Dsg)	Cameroon	Field	Frozen (− 20 °C)	15			2^f	C
Anopheles coluzzii	Dakar (Dkr)	Senegal	Laboratory	Fresh	155 (110)	6^e	6^e	6^e	A, B, C
An. gambie s.s.	Kisumu (Kis)	Kenya	Laboratory	Frozen (− 20 °C)	20		2^f	2^f	C
An. coluzzii	Tibati (Tib)	Cameroon	Field	Silicate, room temperature	16			2^f	C
An. gambie s.s.	Brazaville (Bra)	Congo	Field	Silicate, room temperature	17			2^f	C
Total					408 (120)	12	16	24

DB Database, MP micropipette, PP pellet pestle, TL TissueLyser, RT room temperature
^aThe abbreviation of the name of each strain is given in parentheses
^bNumber of engorged specimens is indicated in parentheses
^cDatabases 1, 2, 3: Number of specimens included in the databases per body part and homogenization mode
^dClassification of parameters assessed according to Fig. 1. A: Effects of homogenization mode and body parts. B: Effect of blood meal. C: Effect of geographical origin
^eTwo specimens per body part homogenized either by MP, PP or TL mode
^fTwo specimens per body part homogenized by TL mode

ISSN: 1756-3305