Table 1 The effect of post-harvest washing, incubation in antiseptic and storage in gentamicin on reducing microbial bioburden on the day of harvest and at 48 h and 7 days following harvest

Sterile water wash

0/3

0/1

Not performed

Betadine 10% 10 min

7/7

Not performed

Not performed

Betadine 1% 10 min

16/16

5/6

0/2

(Larvae in sample)

Betadine 0.1% 10 min

1/1

0/1

Not performed

Betadine 1% 10 min + 1 mg/ml gentamicin

1/1

1/1

2/2

Betadine 1% 10 min + 0.5 mg gentamicin

Not performed

Not performed

1/1

Betadine 1% 10 min + 0.1 mg/ml gentamicin

Not performed

Not performed

1/1

Betadine 1% 10 min + 0.01 mg/ml gentamicin

Not performed

Not performed

0/1

Betadine 1% 10 min + 0.001 mg/ml gentamicin

Not performed

Not performed

0/1

Betadine 1% 10 min, pre-treatment of faeces with amphotericin B and gentamicin

10/10

Not performed

0/2

Betadine 1% 10 min, no pre-treatment of faeces with amphotericin B and gentamicin

7/7

4/5

Not performed

(Larvae in 1 sample)