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Table 4 Cochran's Q test and pairwise McNemar's test for individual and parallel diagnostic methods in the investigation of Dirofilaria immitis in Texas shelter dogs (n = 300)

From: Probe-based qPCR as an alternative to modified Knott’s test when screening dogs for heartworm (Dirofilaria immitis) infection in combination with antigen detection tests

Method combinations

 

P-value

Comparison of individual methods

χ2 = 62.3, df = 3

 < 0.0001

 MK

  

 qPCR

 DiroCHEK® pre-ICD

 DiroCHEK® post-ICD

Post hoc analysis

  

 MK/qPCR

 

0.008

 DiroCHEK® pre-ICD/ DiroCHEK® post-ICD

 

< 0.0001*

Comparison of combined methods

χ2 = 43.1, df = 4

< 0.0001

 MK + DiroCHEK® pre-ICD(A)

  

 MK + DiroCHEK® post-ICD (B)

 qPCR + DiroCHEK® pre-ICD (C)

 qPCR + DiroCHEK® post-ICD (D)

Post hoc analysis

  

 A/B

 

< 0.0001**

 A/C

 

0.157

 B/D

 

1

 C/D

 

0.002**

  1. MK modified Knott’s test; ICD immune complex dissociation; qPCR real-time PCR
  2. * Significant after Bonferroni correction (α = 0.008)
  3. ** Significant after Bonferroni correction (α = 0.005)