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Table 2 Complete blood count results of 7 puppies infected with Hepatozoon canis at day 62 post-partum performed with ADVIA 2120i [Siemens Healthineers] in the Laboklin laboratory (Bad Kissingen, Germany) with age-related reference intervals according to Rortveit et al. (2015)

From: First evidence of vertical Hepatozoon canis transmission in dogs in Europe

Parameter

Reference interval

Puppy I

Puppy II

Puppy III

Puppy IV

Puppy V

Puppy VI

Puppy VII

RBC

4.0–5.5 × 1012/l

4.55

4.71

4.58

4.54

4.39

4.47

5.0

HGB

86–177 g/l

98

109

105

103

100

106

111

HCT

0.27–0.37 l/l

0.32

0.36

0.34

0.32

0.31

0.32

0.36

MCV

63.0–74.0 fl

70.0

77.1

74.0

70.0

72.0

71.0

71.6

MCH

Pga

22.0

23.1

23.0

23.0

23.0

24.0

22.2

MCHC

29.0–34.0 g/dl

31.0

30.0

31.0

33.0

32.0

33.0

31.0

RET

/nla

177.5

203.5

188.2

205.2

180.9

138.6

187.0

CHr

pga

23.0

24.4

23.6

23.5

22.8

26.3

24.0

WBC

8.8–22.4 × 109/l

13.6

20.5

17.3

15.6

18.9

17.3

17.5

Segb

4.1–12.2 × 109/l

6.8

9.8

6.7

7.2

7.9

8.1

11.9

Lymphb

2.7–11.3 × 109/l

4.8

8.6

8.0

7.0

9.1

6.9

3.9

Monob

0.5–1.6 × 109/l

1.4

1.0

1.7

0.9

1.5

0.9

0.9

Eob

0.1–1.8 × 109/l

0.7

1.0

0.7

0.5

0.4

1.4

0.9

Basob

 × 109/l

0.0

0.0

0.0

0.0

0.0

0.0

0.0

Bandsb

 < 0.5 × 109/l

0.0

0.0

0.0

0.0

0.0

0.0

0.0

PLT

193–653 × 109/l

241

399

367

429

280

393

307

Hypochrb

Neg

Neg

Neg

Neg

Neg

Neg

Neg

Neg

Anisob

Neg

Neg

Neg

Neg

Pos

Neg

Neg

Neg

Hepatozoon gamontsc (day 62) (%)

0

4

1

2

1

1.5

1

7

Hepatozoon gamontsc (day 125)

0%

1%

1%

0%

1%

0%

Hepatozoon spp. PCR (day 62)

Negative

Positive (ct 32.8)

Positive (ct 34.8)

Positive (ct 31.8)

Positive (ct 32.6)

Positive (ct 31.1)

Positive (ct 31.5)

Positive (ct 31.8)

Hepatozoon spp. PCR (day 125)

Negative

Positive (ct 34.3)

Negative

Positive (ct 36.1)

Positive (ct 33.1)

Positive (ct 33.0)

  1. Bold values demonstrate parameters out of the diagnostic thresholds
  2. RBC red blood cells; HGB haemoglobin; HCT haematocrit; RET reticulocytes; CHr reticulocyte haemoglobin content; WBC white blood cells; Seg segmented neutrophilic granulocytes; Lymph lymphocytes; Mono monocytes; Eo eosinophilic granulocytes; Baso basophilic granulocytes; Bands banded neutrophilic granulocytes; PLT platelets; Hypochrom hypochromasia; Aniso anisocytosis
  3. aNo reference values provided by Rortveit et al. (2015)
  4. bManual differential count (LABOKLIN GmbH & Co. KG.)
  5. cManual count out of buffy-coat smear (LABOKLIN GmbH & Co. KG.)