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Table 2 Complete blood count results of 7 puppies infected with Hepatozoon canis at day 62 post-partum performed with ADVIA 2120i [Siemens Healthineers] in the Laboklin laboratory (Bad Kissingen, Germany) with age-related reference intervals according to Rortveit et al. (2015)

From: First evidence of vertical Hepatozoon canis transmission in dogs in Europe

Parameter Reference interval Puppy I Puppy II Puppy III Puppy IV Puppy V Puppy VI Puppy VII
RBC 4.0–5.5 × 1012/l 4.55 4.71 4.58 4.54 4.39 4.47 5.0
HGB 86–177 g/l 98 109 105 103 100 106 111
HCT 0.27–0.37 l/l 0.32 0.36 0.34 0.32 0.31 0.32 0.36
MCV 63.0–74.0 fl 70.0 77.1 74.0 70.0 72.0 71.0 71.6
MCH Pga 22.0 23.1 23.0 23.0 23.0 24.0 22.2
MCHC 29.0–34.0 g/dl 31.0 30.0 31.0 33.0 32.0 33.0 31.0
RET /nla 177.5 203.5 188.2 205.2 180.9 138.6 187.0
CHr pga 23.0 24.4 23.6 23.5 22.8 26.3 24.0
WBC 8.8–22.4 × 109/l 13.6 20.5 17.3 15.6 18.9 17.3 17.5
Segb 4.1–12.2 × 109/l 6.8 9.8 6.7 7.2 7.9 8.1 11.9
Lymphb 2.7–11.3 × 109/l 4.8 8.6 8.0 7.0 9.1 6.9 3.9
Monob 0.5–1.6 × 109/l 1.4 1.0 1.7 0.9 1.5 0.9 0.9
Eob 0.1–1.8 × 109/l 0.7 1.0 0.7 0.5 0.4 1.4 0.9
Basob  × 109/l 0.0 0.0 0.0 0.0 0.0 0.0 0.0
Bandsb  < 0.5 × 109/l 0.0 0.0 0.0 0.0 0.0 0.0 0.0
PLT 193–653 × 109/l 241 399 367 429 280 393 307
Hypochrb Neg Neg Neg Neg Neg Neg Neg Neg
Anisob Neg Neg Neg Neg Pos Neg Neg Neg
Hepatozoon gamontsc (day 62) (%) 0 4 1 2 1 1.5 1 7
Hepatozoon gamontsc (day 125) 0% 1% 1% 0% 1% 0%
Hepatozoon spp. PCR (day 62) Negative Positive (ct 32.8) Positive (ct 34.8) Positive (ct 31.8) Positive (ct 32.6) Positive (ct 31.1) Positive (ct 31.5) Positive (ct 31.8)
Hepatozoon spp. PCR (day 125) Negative Positive (ct 34.3) Negative Positive (ct 36.1) Positive (ct 33.1) Positive (ct 33.0)
  1. Bold values demonstrate parameters out of the diagnostic thresholds
  2. RBC red blood cells; HGB haemoglobin; HCT haematocrit; RET reticulocytes; CHr reticulocyte haemoglobin content; WBC white blood cells; Seg segmented neutrophilic granulocytes; Lymph lymphocytes; Mono monocytes; Eo eosinophilic granulocytes; Baso basophilic granulocytes; Bands banded neutrophilic granulocytes; PLT platelets; Hypochrom hypochromasia; Aniso anisocytosis
  3. aNo reference values provided by Rortveit et al. (2015)
  4. bManual differential count (LABOKLIN GmbH & Co. KG.)
  5. cManual count out of buffy-coat smear (LABOKLIN GmbH & Co. KG.)