Fig. 6

Effects of PERK and caspase-12 inhibitors on loss of cell viability and apoptosis in pEGFP-GRA3_wh6 transfected N2a cells. N2a cells were treated with or without GSK2656157 (4 μM) and Z-ATAD-FMK (ZAF, 5 μM) for 1.5 h and 6 h, respectively, and transfected with either pEGFP or pEGFP-GRA3_Wh6 (GRA3_Wh6) for 24 h. a Cell viability was measured using the trypan blue staining cell viability assay. b Apoptosis of cells was determined using flow cytometry after staining with Annexin V-PE/7-AAD. The plots are from a representative measurement and the data were expressed as mean ± SD on three different assays (n = 3). c The protein expression levels of ER stress- and apoptosis-related proteins were determined by immunoblotting. The represented values were normalized and expressed relative to β-actin levels. The data were expressed as mean ± SD on three different assays (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, and ns, not statistically significant. GRA3_Wh6 + GSK2656157 represents N2a cells pretreated with GSK2656157, followed by transfection with GRA3_Wh6 plasmid. GRA3_Wh6 + ZAF represents N2a cells pretreated with ZAF, followed by transfection with GRA3_Wh6 plasmid