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Fig. 1 | Parasites & Vectors

Fig. 1

From: Histidine acid phosphatase domain-containing protein from Haemonchus contortus is a stimulatory antigen for the Th1 immune response of goat PBMCs

Fig. 1

Cloning, expression and western blot analysis of Hc-HAP. a Amplification of Hc-HAP. Lanes: M DNA marker DL 2000, 1 amplification products of the Hc-HAP gene. b Lanes: M DNA marker DL10000, 1 digestion of pET-32a/Hc-HAP plasmid by enzymes. c–f Expression and purification of rHc-HAP and pET-32a. Lane: M Standard protein molecular weight marker. c Lanes: 1, 2 pET-32a induced by IPTG for 0 and 5 h, 3–8 pET-32a/Hc-HAP induced by IPTG for 0 to approx. 5 h. d Lanes: 1 Supernatant of expression products, 2 inclusion body of expression products. e Lane: 1 purification of rHc-HAP. f Lane: 1 purification of pET-32a. g Western blot analysis of rHc-HAP protein. Lanes: M Standard protein molecular weight marker, 1 rHc-HAP detected by serum incubated with positive H. contortus goat, 2 no protein was detected with normal goat sera. Abbreviations: Hc-HAP, histidine acid phosphatase gene from H. contortus; IPTG, isopropyl-β-D-thiogalactopyranoside; rHC-HAP, purified recombinant Hc-HAP

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Parasites & Vectors

ISSN: 1756-3305

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