Fig. 8
Effect of rHc-HAP on NO secretion from goat PBMCs. Cells were incubated with serial concentrations of rHc-HAP and pET-32a for 24 h at 37 °C and 5% CO2. a The effect of rHc-HAP on NO release from PBMCs was examined using a commercial kit. b The effect of rHc-HAP on the expression level of iNOS in PBMCs was analyzed using western blot assays. Data are presented as the mean ± SEM from three independent experiments. Asterisks indicate significant difference at *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs the control group. Abbreviations: iNOS, Iducible nitric oxide synthase; NO nitric oxide