Fig. 9

To assess the effect of rHc-HAP on the transcriptional expression of IFN-γ, IL-4, IL-9, IL-17 and p-STAT1 in goat PBMCs. a The transcript levels of IFN-γ, IL-4, IL-9, and IL-17 were detected by qPCR assays. b The expression levels of IFN-γ, IL-4, IL-9, IL-17, p-STAT1, and STAT1 were detected by western blot assays. Data are presented as the mean ± SEM from three independent experiments. Asterisks indicate significant differences at *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs the control group. Abbreviations: IFN, Interferon; IL, interleukin; p-STAT1, phosphorylated signal transducer and activator of transcription factor