Fig. 11

Theileria annulata SVSP455 partially rescues HSP60 and host cell apoptosis-related gene expression in T. annulata-transformed cells. A Myc-tagged SVSP455 partially rescued HSP60 protein levels upon Bup treatment. B, C T. annulata partially rescued expression of host cell genes for mitochondrial apoptosis signaling upon Bup treatment and overexpression SVSP455 + Bup treatment using qPCR (B) and immunoblotting (C). Bovine β-actin mRNA was used for normalization. A mouse anti-α-tubulin monoclonal antibody was used as the loading control. Con: Control; Bup: Buparvaquone; Bup + SVSP455: T. annulata-transformed cells transfected with recombinant plasmid Myc-tagged SVSP455 and treated with Bup; qPCR: quantitative PCR. Asterisks indicate significant difference at *P < 0.05, **P < 0.01 and ***P < 0.001. p Proteins involved in cell apoptosis signaling:  p53, BCL-2, BCL-XL, MCL-1, SURVIVIN, BAX, BAD and Cytochrome c