Fig. 7
SVSP455 directly interacts with bovine HSP60 in the cell. A, B The MFI of BiFC green-fluorescent signals was analyzed using a flow cytometry assay and the results are presented as MFI values relative to those of signals of the untransfected cells, which were used as the negative control. C The subcellular colocalization of the red signals for VN-SVSP455 and the BiFC green fluorescence signals of interacting pairs was observed using confocal microscopy. Scale bar: 25 μm. Asterisks indicate significant difference at *P < 0.05, **P < 0.01 and ***P < 0.001. Abbreviations: BiFC: Bimolecular fluorescence complementation; MFI: mean fluorescence intensity