Fig. 3

Differences in T. gondii parasite invasion ability do not affect the strain-specific activation of NF-κB promoter. HEK 293 T cells were infected with T. gondii for 18 h and fixed; the extracellular and intracellular parasites were distinguished by differential IFA staining. a The overall parasite invasion rate for each strain in HEK293T cells. The percentage of infection was determined by counting at least 15 HEK293T cells for each strain at each MOI. b MOI for different parasites was adjusted to make comparative parasite invasion rate in HEK293T cells; 18 h post infection, the luciferase activity was measured in cell lysates. Data are presented as the mean relative luciferase intensity with standard deviation of three independent experiments (^****P < 0.0001 and NS not significant)