Structural analysis of PpSP15 and PsSP9 sand fly salivary proteins designed with a self-cleavable linker as a live vaccine candidate against cutaneous leishmaniasis

Table 1 RNAfold prediction results for all different combinations

Constructs	MFE of the optimal secondary structure (kcal/mol)	Ensemble diversity^a	MFE of the centroid secondary structure^b (kcal/mol)	The free energy of thermodynamic ensemble^c (kcal/mol)
PpSP15-T2A-PsSP9	−399.80	262.68	−329.73	−423.73
PpSP15–PsSP9	−317.50	274.74	−255.10	−339.65
PsSP9-T2A-PpSP15	−402.60	402.39	−265.50	−424.09
PsSP9–PpSP15	−323.50	346.15	−186.30	−342.44

MFE: minimum free energy
^aIs the average base-pair distance between all structures in the Boltzmann ensemble
^bMFE of the structure with minimal base-pair distance to all structures in the thermodynamic ensemble
^cIs a statistical ensemble in statistical equilibrium that prepares a way to infer the properties of a real thermodynamic system

ISSN: 1756-3305