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Table 2 Comparison of genetic markers (ITS and gltA) used to detect Bartonella species in Arctic and subarctic rodents and shrews in NT, Canada, via conventional PCR

From: Combining deep sequencing and conventional molecular approaches reveals broad diversity and distribution of fleas and Bartonella in rodents and shrews from Arctic and Subarctic ecosystems

Year

Species (#)

Positives with ITS region (% [95% CI])

Positives with gltA gene (% [95% CI])

Positives with both (% [95% CI])

2017

Northern Red-backed vole (18)

Deer Mouse (9)

Meadow Vole (3)

Shrews (15)

3 (17)

2 (22)

0 (0)

5 (15)

2 (11)

0 (0)

0 (0)

1 (7)

2 (11)

0 (0)

0 (0)

1 (7)

2017 Small mammal total

45

10 (22 [13–36])

3 (7 [2–18])

3 (7 [2–18])

2018

Northern red-backed vole (168)

Deer Mouse (41)

Meadow Vole (12)

Collared Lemming (8)

Shrews (44)

40 (24)

15 (37)

4 (33)

0 (0)

13 (30)

11 (7)

0 (0)

0 (0)

0 (0)

3 (7)

9 (5)

0 (0)

0 (0)

0 (0)

3 (7)

2018 Small mammal total

273

72 (26 [22–32])

14 (5 [3–8])

12 (4 [3–8])

2019

Northern red-backed vole (163)

Deer Mouse (18)

Meadow Vole (5)

Collared Lemming (1)

77 (47)

8 (44)

3 (60)

0 (0)

62 (38)

0 (0)

0 (0)

0 (0)

45 (28)

0 (0)

0 (0)

0 (0)

2019 Small mammal total

187

88 (47 [40–54])

62 (33 [27–40])

45 (24 [19–31])

Project total

505

170 (34 [30–38])

79 (16 [13–19])

60 (12 [9–15])