Table 4 Comparing rodents collected in NT, Canada (2017–2019), that underwent Sanger sequencing following conventional PCR (ITS region) and metagenomic sequencing following modified gltA PCR. Only amplicon sequence variants accounting for at least 1% in a sample were included
Location | Rodent species1 | Bartonella species (Sanger sequencing) | % Identity | Accession number (ITS) | Bartonella species (metagenomic sequencing) | % Identity | Accession number (gltA) | Amplicon sequence variants |
|---|---|---|---|---|---|---|---|---|
| Â | RBV | Unidentified | 91 | KX420734 | B. heixiaziensis | 98 | KJ175047 | NT-heix-1 NT-heix-4 |
Fort Resolution | RBV | B. grahamii | 99 | MH687377 | B. grahamii | 99 | NC_012846 | NT-grah-1 |
| Â | RBV | B. elizabethae | 100 | LR134527 | B. heixiaziensis | 99 | KJ175047 | NT-heix-3 NT-heix-6 |
| Â | RBV | B. grahamii | 99 | MH687377 | B. grahamii | 99 | NC_012846 | NT-grah-1 |
| Â | DM | Unidentified | 90 | KU292577 | B. vinsonii | 99 | AF214557 | NT-vins-1 NT-vins-2 NT-vins-3 |
| Â | RBV | B. grahamii | 99 | MH547345 | B. heixiaziensis | Â | Â | NT-heix-1 NT-heix-4 |
Yellowknife | RBV | Failed | Failed | Failed | B. heixiaziensis | Â | Â | NT-heix-1 NT-heix-4 |
| Â | RBV | B. vinsonii | 99 | AF167988 | B. heixiaziensis | 98 | KJ175047 | NT-heix-1 NT-heix-4 |
| Â | RBV | Candidatus B. rudakovii | 100 | EF682087 | B. heixiaziensis | Â | Â | NT-heix-1 NT-heix-4 |
| Â | RBV | Unidentified | 96 | KX420719 | B. heixiaziensis | Â | Â | NT-heix-1 NT-heix-4 |
Fort Smith | RBV | Unidentified | 95 | DQ683199 | Candidatus B. rudakovii | 98 | EF682090 | NT-ruda-1 |
| Â | RBV | Unidentified | 94 | KX420620 | Candidatus B. rudakovii | Â | Â | Â |
Fort | RBV | Failed | Failed | Failed | B. grahamii | 99 | NC_012846 | NT-grah-1 |
Simpson | RBV | B. grahamii | 98 | MH687377 | B. grahamii | Â | Â | Â |
| Â | RBV | Unidentified | 94 | AB602558 | B. heixiaziensis | Â | Â | NT-heix-2 NT-heix-5 |
| Â | RBV | Unidentified | 87 | AB602558 | B. heixiaziensis | Â | Â | NT-heix-2 NT-heix-5 |
Inuvik | RBV | Unidentified | 88 | MH477609 | B. heixiaziensis | 99 | KJ175047 | NT-heix-2 NT-heix-5 |
| Â | RBV | Unidentified | 94 | AB602558 | B. heixiaziensis | 98 99 99 | KJ175047 | NT-heix-1 NT-heix-2 NT-heix-5 |
| Â | RBV | B. grahamii | 99 | AJ269785 | Â | Â | Â | NT-grah-1 |
| Â | RBV | B. grahamii | 97 | CP001562 | Â | Â | Â | NT-grah-1 NT-grah-2 |
| Â | RBV | B. grahamii | 99 | MH687377 | Â | Â | Â | NT-grah-1 |
| Â | RBV | B. grahamii | 98 | MH547345 | Â | Â | Â | NT-grah-1 |
| Â | RBV | B. grahamii | 99 | MH547345 | Â | Â | Â | NT-grah-1 |
| Â | RBV | B. grahamii | 98 | MH687377 | Â | Â | Â | NT-grah-1 |
| Â | RBV | Failed | Failed | Failed | Â | Â | Â | NT-grah-1 |
| Â | RBV | Failed | Failed | Failed | Â | Â | Â | NT-grah-1 |
| Â | RBV | B. grahamii | 99 | MH687377 | Â | Â | Â | NT-grah-1 NT-grah-2 |
| Â | RBV | Failed | Failed | Failed | Â | Â | Â | NT-grah-2 |
| Â | RBV | B. grahamii | 98 | MH687377 | Â | Â | Â | NT-grah-1 NT-grah-2 |
| Â | RBV | B. grahamii | 97 | MH547345 | Â | Â | Â | NT-grah-1 |
Fort Liard | RBV | B. grahamii | 99 | MH547345 | B. grahamii | 99 | NC_012846 | NT-grah-1 |
| Â | RBV | B. vinsonii | 90 | MK773863 | Â | Â | Â | NT-grah-1 |
| Â | RBV | Unidentified | 95 | AJ269785 | Â | Â | Â | NT-grah-1 NT-grah-2 |
| Â | RBV | B. grahamii | 99 | MH547345 | Â | Â | Â | NT-grah-3 |
| Â | RBV | Failed | Failed | Failed | Â | Â | Â | NT-grah-2 |
| Â | RBV | Failed | Failed | Failed | Â | Â | Â | NT-grah-1 NT-grah-2 |
| Â | RBV | Failed | Failed | Failed | Â | Â | Â | NT-grah-1 |
| Â | RBV | Failed | Failed | Failed | Â | Â | Â | NT-grah-1 |
| Â | RBV | B. grahamii | 98 | MH547345 | Â | Â | Â | NT-grah-1 NT-grah-2 |
| Â | RBV | Failed | Failed | Failed | Â | Â | Â | NT-grah-1 |
| Â | RBV | B. grahamii | 97 | AJ269785 | Â | Â | Â | NT-grah-2 |
| Â | RBV | Failed | Failed | Failed | Â | Â | Â | NT-grah-1 NT-grah-2 |