Fig. 3

Dysfunction in dNK cells in infected 2B4^−/− pregnant mice leads to increased TNF-α and IFN-γ expression. A CD69 expression in infected WT mice and infected 2B4^−/− mice was detected by flow cytometry (data are presented as the mean ± SD, n = 3, *P < 0.05, by unpaired t-test). B, F Percentage of TNF-α⁺CD45⁺CD3⁻CD122⁺CD49b⁻ dNK cells in uninfected mice (n = 7), infected WT mice (n = 14), and infected 2B4^−/− mice (n = 7) (data are presented as the mean ± SD, **P < 0.01, *P < 0.05, by unpaired t-test). E Percentage of TNF-α⁺CD45⁺CD3⁻CD122⁺CD49b⁺ dNK cells in uninfected mice (n = 7), infected WT mice (n = 17), and infected 2B4^−/− mice (n = 9) (data are presented as the mean ± SD; P > 0.05 [WT-uninfected vs. WT-infected], P > 0.05 [WT-infected vs. 2B4^−/−infected], by the unpaired t-test). C, G IFN-γ expression in the CD45⁺CD3⁻CD122⁺CD49b⁻dNK subset from uninfected mice (n = 7), infected WT mice (n = 10), and infected 2B4^−/− mice (n = 8) (data are presented as the mean ± SD; *P < 0.05 [WT-uninfected vs. WT-infected], *P < 0.05 [WT-infected vs. 2B4^−/−−infected], by unpaired t-test). D, H IFN-γ in CD45⁺CD3⁻CD122⁺CD49b⁺ dNK cell subset from uninfected WT mice (n = 9), infected WT mice (n = 10), and infected 2B4^−/− mice (n = 8). Data are presented as the mean ± SD; *P < 0.05 (WT-uninfected vs. WT-infected), *P < 0.05 (WT-infected vs. 2B4^−/−infected), by unpaired t-test