Fig. 4

Downregulation of 2B4 and p-2B4 is associated with human dNK cell dysfunction during T. gondii infection. A, B PBMCs were isolated from healthy abortions from early pregnancy and stimulated with T. gondii for 20 h. 2B4 expression on the surface of dNK cells in the uninfected and infected groups (data are presented as the mean ± SD, n = 7, *P < 0.05, by the paired t-test). C Purified dNK cells were identified by flow cytometry. D 7-AAD was used to excluded the influences of dead cells. E h2B4-A and h2B4-B mRNA levels in uninfected and infected dNK cells. F Western blot analysis of 2B4 and p-2B4 in uninfected and infected dNK cells (data are presented as the mean ± SD, n = 3, *P < 0.05, by the paired t-test). G Expression of 2B4 ligand CD48 on dNK cells (data are presented as the mean ± SD, n = 3, **P < 0.01, by the paired t-test). H Immunofluorescence shows mean fluorescence intensity of 2B4 in uninfected and infected dNK cells (data are presented as the mean ± SD, **P < 0.01, by the paired t-test). I CD107a expression in uninfected and infected dNK cells with or without anti-2B4 antibody (data are presented as the mean ± SD, *P < 0.05, by the paired t-test). J LDH release in uninfected and infected dNK cells with or without anti-2B4 antibody