Table 2 Antibodies used for western blot assays

α-GCN1 primary—rabbit

293

1:1000

Abcam (ab86139)

α-eIF2α primary—mouse

36

1:250

Abcam (ab5369)

α-phospho-eIF2α primary—rabbit

36

1:250

Abcam (ab32157)

α-actin primary—mouse

43

1:500

MilliporeSigma (MAB1501)

α-mouse HRP secondary

N/A

1:1000 or 1:2000 (actin)

MilliporeSigma (12–349)

α-rabbit HRP secondary

N/A

1:1000

MilliporeSigma (12–348)