Fig. 6

Cellular localization of the P30 protein in B. gibsoni. a During the extracellular stage, BgP30 with green fluorescence co-located with the nucleus of merozoites with blue fluorescence. b When the merozoite resided in the red blood cell, the poly-antibody against rBgP30 detected P30 in the intraerythrocytic merozoite of B. gibsoni. c At the post-invasion stage of paired parasites, P30 was also detected with green fluorescence adjacent to the merozoite nucleus. d Negative control: infected erythrocytes were incubated with the serum of B. gibsoni-naïve rabbits. Scale bar: 1 μm