Table 1 Primer sequences for RPA, crRNA and CRISPR-Cas12a
Assay | Primer name | Sequence (5′ → 3′) |
|---|---|---|
Target | TV1 | TTTCCCTCTACTCCTCTGGCCGTA |
TV2 | TTTCGATGCTGGTGATGGTGTTTC | |
TV3 | TTTCCCATCCGTTGTTGGCCGTCC | |
RPA | RPA-F1 | CCAAAGGCTAACCGTGAGAAAATGA |
RPA-F2 | CATTCAACGCCCCATCCTTCTATGTCGG | |
RPA-F3 | GGCTGTTCTTTCCCTCTACTCCTCTGGC | |
RPA-F4 | AACCCAAAGGCTAACCGTGAGAAAATGAT | |
RPA-F5 | TCCAAGGCTGGTGTCCTCATCCTCAAGTA | |
RPA-F6 | TAACCCAAAGGCTAACCGTGAGAAAATGA | |
RPA-F7 | GCTCCAAGGCTGGTGTCCTCATCCTCAAG | |
RPA-R1 | GAAGTATGGCTTGAAGAGCATTTCTGGGC | |
RPA-R2 | TAGCCTTCGTAAATTGGAACTGTGTGGGAAAC | |
RPA-R3 | GGAGTAGCCTTCGTAAATTGGAACTGTGTGGG | |
RPA-R4 | GGCTGTTGTGTTGAAAGCATTGCCACGCTCTGTG | |
crRNA | TV1-crRNA | GAAATTAATACGACTCACTATAGGGTAATTTCTACTAAGTGTAGATCCTCTACTCCTCTGGCCGTA |
TV2-crRNA | GAAATTAATACGACTCACTATAGGGTAATTTCTACTAAGTGTAGATGATGCTGGTGATGGTGTTTC | |
TV3-crRNA | GAAATTAATACGACTCACTATAGGGTAATTTCTACTAAGTGTAGATCCATCCGTTGTTGGCCGTCC | |
Cas12a | Cas12a-pGEX-4T-1-F | CCGCGTGGATCCCCGGAATTCATGAGCAAGCTGGAGAAGTTTACA |
Cas12a-pGEX-4T-1-R | TCAGTCAGTCACGATGCGGCCGCGTGCTTCACGCTGGTCTGGG |