Fig. 6

Injection with AgB altered the immune status of mice with DSS-induced colitis. LPMCs were collected from mouse intestines and tested by flow cytometry. Live cells were gated on CD45 and F4/80 double expression to identify macrophages; then, FITC-labelled CD11 expression was used to identify M1 cells, and APC-labelled CD206 expression was used to identify M2 cells. Comparison between groups was performed to determine the following: A There was no significant difference in the percentage of macrophages between the PBS+DSS group and other groups (n = 6–10). B The percentage of M1 cells between the groups also showed no significant difference (n = 6–10). C The percentage of M2 cells in the PBS+DSS group was reduced by 11.49 ± 2.95% compared with that in the AgB+DSS group (44.28 ± 6.04%, n = 6–10, P < 0.05). D The ratio of M1/M2 cells. Comparison between groups was performed using one-way analysis of variance (ANOVA) with Bonferroni multiple comparison post-test for statistical analysis (the experiment was repeated independently (n = 6–10). Bars represent the mean ± SD, ∗ P < 0.05, ∗ ∗ P < 0.01, ∗ ∗ ∗ ∗ P < 0.0001)