Fig. 10

a–h Mapping HSP 40 in Schistosoma mansoni. Cercariae, somules, male and female adult worms were fixed and processed for immunofluorescence using anti-HSP 40 primary and Alexa Fluor 488 secondary antibodies (green); rhodamine phalloidin (red) was used to stain filamentous actin. Samples were mounted on slides and images captured on a Zeiss LSM 800 confocal microscope. HSP 40 localised in a, b cercariae; c 3-h somules; d 24-h somules; e–g adult males; and h adult females. Representative micrographs are single z-sections through the parasite. Scale bars = 25 µm (for cercariae and somules) and 50 µm (for adult worms)