Fig. 11

a–g Mapping HSP 70 in Schistosoma mansoni. Cercariae, somules, and male and female adult worms were fixed and processed for immunofluorescence using anti-HSP 70 primary and Alexa Fluor 488 secondary antibodies (green); rhodamine phalloidin (red) was used for filamentous actin staining. Samples were mounted on slides and images captured using a Zeiss LSM 800 confocal microscope. HSP 70 localised in a cercariae; b 3-h somules; c 24-h somules; d, e adult males; and f adult females. Representative images are e–g maximum projections of confocal z-stacks; the rest are single z-sections through the parasite. Scale bars = 25 µm (for cercariae and somules) and 50 µm (for adult worms)