Fig. 12

a–h Mapping of HSP 90 in Schistosoma mansoni. Cercariae, somules, male and female adult worms were fixed and processed for immunohistochemistry using anti-HSP 90 primary and Alexa Fluor 488 secondary antibodies (green); rhodamine phalloidin (red) was used for filamentous actin staining. Samples were mounted on slides and images captured on a Zeiss LSM 800 confocal microscope. HSP 90 localised in the a cercariae; b 3-h somules; c 24-h somules; d–f adult males; and g, h adult females. Representative micrographs are single z-sections through the parasite. Scale bars = 25 µm (for cercariae and somules) and 50 µm (for adult worms)