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Fig. 7 | Parasites & Vectors

Fig. 7

From: Molecular insights into the heat shock proteins of the human parasitic blood fluke Schistosoma mansoni

Fig. 7

Detection of HSPs in Schistosoma mansoni. In vitro 24-h-cultured somules (~ 1000 per lane) or adult worms (one male or female per lane) were homogenised and protein extracts subjected to western blotting with one of the following antibodies: anti-HSPE1 (ARPP54651_P050) (HSP 10) raised against a synthetic peptide located within amino acid 55–102 of human HSPE1; anti-HSP 40 (SPC-100), which recognises the whole amino acid sequence of human DNAJB1; anti-HSP 60 (SMC-111A/B) raised against amino acid sequence 382–447 of human HSP 60; anti-HSP 70 (SMC-162 C/D) raised against the whole amino acid sequence of human heat shock 70 kDa protein 1A and heat shock 70 kDa protein 1B; or anti-HSP 90 (STJ93608) that recognises amino acids 180–260 of human HSP 90β

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