Fig. 9

a–g Mapping HSP 60 in Schistosoma mansoni. Cercariae, somules, male and female adult worms were fixed and processed for immunofluorescence using anti-HSP 60 primary and Alexa Fluor 488 secondary antibody (green) antibodies; rhodamine phalloidin (red) was used to stain filamentous actin. Samples were mounted on slides and images captured on a Zeiss LSM 800 confocal microscope. HSP 60 localised in a, b cercariae; c 3-h somules; d 24-h somules; e, f adult males; and g adult females. Representative micrographs are single z-sections through the parasite. Scale bars = 25 µm (for cercariae and somules) and 50 µm (for adult worms)