First genetic report of Ixodes kashmiricus and associated Rickettsia sp.

Parasites & Vectors

Table 1 List of primers used for the amplification of targeted gene sequences of Ixodes tick and associated rickettsial species

Organism/gene	PCR	Primer	Sequence 5' to 3'	Amplicon size	References
Tick/16S rRNA	PCR	16S + 1	CCGGTCTGAACTCAGATCAAGT	460 bp	[40]
Tick/16S rRNA	PCR	16S-1	GCTCAATGATTTTTTAAATTGCTG	460 bp	[40]
Tick/ITS2	PCR	5.8S	CCATCGATGTGAAYTGCAGGACA	800 bp	[41]
Tick/ITS2	PCR	28S	GTGAATTCTATGCTTAAATTCAGGGGT	800 bp	[41]
Rickettsia spp./gltA	RT-PCR	CS-5	GAGAGAAAATTATATCCAAATGTTGAT	147 bp	[42]
Rickettsia spp./gltA	RT-PCR	CS-6	AGGGTCTTCGTGCATTTCTT	147 bp	[42]
Rickettsia spp./gltA	PCR	CS-78	GCAAGTATCGGTGAGGATGTAAT	401 bp	[42]
Rickettsia spp./gltA	PCR	CS-323	GCTTCCTAAAATTCAATAAATCAGGAT	401 bp	[42]
Rickettsia spp./ompA	PCR	Rr190.70	ATGGCGAATATTTCTCCAAAA	631 bp	[43]
Rickettsia spp./ompA	PCR	Rr190.701	GTTCCGTTAATGGCAGCATCT	631 bp	[43]

ISSN: 1756-3305