Fig. 3

Affinity purification results of the recombinant proteins separated by passage through a nickel column. A Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) analysis of purified recombinant protein HSC70 (rHSC70). B SDS-PAGE analysis of purified recombinant protein HSC70^TKD (rHSC70^TKD). C Identification of rHSC70 and rHSC70^TKD by western blot. Lanes: M, Protein marker; 1, 9, the supernatant of rHSC70 and rHSC70^TKD after bacterial destruction, respectively; 2, 10, rHSC70 and rHSC70^TKD passing through the column, respectively; 3, 4 and 11–13, rHSC70 and rHSC70^TKD 20 mmol/l eluent, respectively; 5–8 and 14–17, rHSC70 and rHSC70^TKD 200 mmol/l eluent, respectively; a. purified rHSC70; b. purified rHSC70^TKD