Fig. 3

Indole and insect repellents (DEET, IR3535) increased the baseline current state of AaegOR8-Orco-injected oocytes. a Representative current traces of AaegOR8-Orco successively exposed to 100 nM (R)-1-octen-3-ol in ND96 buffer (saline; black trace) and continuous perfusion under indole (0.005 M), DEET (0.01 M) and IR3535 (0.01 M) perfusions (red traces), with increasing concentrations of (R)-1-octen-3-ol, ND96 buffer perfusion and 100 nM (R)-1-octen-3-ol. The same protocol was also applied to water-injected oocytes. In AaegOR8-Orco-injected oocytes, perfusion buffer transitions from ND96 buffer to indole, DEET or IR3535 are shown as solid red arrows, while the transitions in the reverse direction are shown as a solid black arrow. In water-injected oocytes, the corresponding buffer transitions are indicated by empty red and black arrows. The baseline currents of OR8-Orco-injected oocytes exhibited significant increases and decreases compared to those of water-injected oocytes. Statistical significance was determined using the Wilcoxon–Mann–Whitney test (n = 5–9). b Under saline perfusion (ND96), AaegOR8-Orco elicitd a stable current baseline. A stimulus pulse of (R)-1-octen-3-ol activated AaegOR8-Orco, which translated into a transient whole-cell depolarization current. A pulse of (R)-1-octen-3-ol (low concentration) and indole (high concentration) mixture transiently induced a hyperpolarization current. Increasing (R)-1-octen-3-ol concentrations reversed indole blockade. High indole concentrations elicited small depolarization and hyperpolarization currents. Under indole perfusion, the current baseline was increased. IR3535, Ethyl butylacetylaminopropionate