Fig. 2

Structural domains of P. vivax RAMA protein (PvRAMA) and design of primers. a Primary structure of PvRAMA protein. b Design of pvrama amplification primers. Gray boxes indicate exons, black lines indicate introns. c, d Amplification of full-length and two fragments of pvrama by semi-nested PCR (one isolate as an example). Arrowheads indicate the bands at the right size. Lanes: M, DNA marker; 1, full-length pvrama (3149 bp); 2, first fragment of pvrama (1922 bp); 3, second fragment of pvrama (1849 bp). aa, Amino acids; pvrama, P. vivax rama gene; RAMA, rhoptry-associated membrane antigen