Table 1 Bovine sera selected for the present study from a serum panel previously used to validate the in-house Bb-cELISA1 [11]
Herd (number of herds) | Tests used to characterize sera | Status | Number (additional information) | Number of sera used for estimates of diagnostic sensitivity and specificity (reference) |
|---|---|---|---|---|
Besnoitiosis herds (n = 2) | B. besnoiti tachyzoite IFAT, tachyzoite immunoblot, bradyzoite immunoblot, BbAPureELISA and Bb-cELISA1 | Besnoitia positive | 95 (positive IFAT titre, 1:100 [n = 19], 1:200 [n = 20], 1:400 [n = 18], 1:800 [n = 10], 1:1600 [n = 10], 1:3200 [n = 10], 1:6400 [n = 4]) | 95 (reference positive) |
Besnoitia negative | 9 (positive IFAT titre, 1:100 [n = 7], 1:200 [n = 1]) | 8 (reference inconclusive), 1 (reference negative) | ||
Neosporosis herd (n = 1) | N. caninum p38 ELISA, N. caninum p38 avidity ELISA | Neospora positive | 97 (including 6 with abortion) | 97 (reference negative) |
Neospora negative | 4 | 4 (reference negative) | ||
Sarcocystosis herd (n = 1) | Serologically not confirmed | Sera from suspected Sarcocystis spp. infectionsa | 100 | 100 (reference negative) |