Fig. 4

Heatmap representing the average nucleotide identity blast (ANIb) values of the 214 minicircle sequences of L. martiniquensis strain PCM3 compared with 40 minicircle sequences of other Leishmania species and Herpetomonas samuelpessoai (a). The ANIb value was obtained by the pyani tools in the blastn alignment mode. The heatmap was created using the Heatmaply program and the basic ANIb identity value received from the pyani package output. The red box area in (a) was enlarged in (b) to show detail of the 53 minicircle sequences of L. martiniquensis strain PCM3 that were highly matched to all 40 Leishmania minicircles from the database. The purple box area in (a) was expanded in (c) to show the remaining minicircles that were variably compared to those of other Leishmania species