Table 1 Internal and external validation of the identification of Schistosoma specimens stored in different media [70% (v/v) ethanol, RNAlater], using a newly expanded in-house database, implemented in MBT Compass Explorer software (Bruker Daltonics, Bremen, Germany)
Internal validation | |||||
|---|---|---|---|---|---|
Species | Preservation media | Number of samples | Identification results | ||
Number of spectra | LSV ≥ 1.7 | LSV ≥ 2.0 | |||
Mixed adults (males/females) | Mixed adults (males/females) | ||||
Schistosoma mansoni | Ethanol | 1 | 31 | 31/31 (100%) | 31/31 (100%) |
RNAlater | 1 | 27 | 27/27 (100%) | 27/27 (100%) | |
Schistosoma japonicum | Ethanol | 1 | 29 | 29/29 (100%) | 29/29 (100%) |
RNAlater | 1 | 32 | 32/32 (100%) | 32/32 (100%) | |
Total | 4 | 119 | 119/119 (100%) | 119/119 (100%) | |
External validation | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Species | Preservation media | Number of samples | Identification results | |||||||
LSV ≥ 1.7 | LSV ≥ 2.0 | |||||||||
Mixed adults (males/females) | Females | Males | % Correct | Mixed adults (males/females) | Females | Males | % Correct | |||
S. mansoni | Ethanol | 18 | 6/6 (100%) | 6/6 (100%) | 6/6 (100%) | 18/18 (100%) | 6/6 (100%) | 5/6 (83.3%) | 6/6 (100%) | 11/18 (61.1%) |
RNAlater | 20 | 10/10 (100%) | 5/5 (100%) | 5/5 (100%) | 20/20 (100%) | 10/10 (100%) | 4/5 (80%) | 5/5 (100%) | 19/20 (95%) | |
S. japonicum | Ethanol | 10 | 4/4 (100%) | 3/3 (100%) | 3/3 (100%) | 10/10 (100%) | 4/4 (100%) | 0/3 (0%) | 3/3 (100%) | 7/10 (70%) |
RNAlater | 10 | 4/4 (100%) | 3/3 (100%) | 3/3 (100%) | 10/10 (100%) | 4/4 (100%) | 3/3 (100%) | 3/3 (100%) | 10/10 (100%) | |
Total | 58 | 26/26 (100%) | 17/17 (100%) | 17/17 (100%) | 58/58 (100%) | 24/24 (100%) | 12/17 (70.6%) | 11/17 (64.7%) | 47/58 (81%) | |