Table 1 The number of individuals (and successful amplifications) for both species of Culicoides that underwent PCR amplification by time point (h) and storage method
Time point (h) | Culicoides nubeculosus | Culicoides nubeculosus | Culicoides sonorensis | Culicoides sonorensis |
|---|---|---|---|---|
Ambient | − 20 °C Freezer | Ambient | − 20 °C Freezer | |
0 | 20 (20) | 20 (19) | 20 (19) | 20 (20) |
8 | 15 (15) | 15 (15) | 15 (15) | 15 (15) |
16 | 15 (15) | 15 (15) | 15 (15) | 15 (15) |
24 | 15 (14) | 15 (14) | 15 (14) | 15 (14) |
32 | 15 (14) | 15 (13) | 15 (10) | 15 (9) |
40 | 15 (4) | 15 (9) | 15 (4) | 15 (3) |
48 | 15 (4) | 15 (6) | 15 (4) | 15 (5) |
56 | 15 (2) | 15 (2) | 15 (3) | 15 (1) |
64 | 15 (9) | 15 (4) | 13 (0) | 12 (0) |
72 | 15 (2) | 15 (1) | 15 (3) | 15 (4) |
80 | 15 (3) | 15 (1) | 8 (0) | 7 (2) |
88 | 15 (4) | 15 (3) | 12 (2) | 10 (0) |
96 | 15 (0) | 15 (2) | 7 (1) | 7 (1) |