Fig. 3

TgCtwh6 infection increases APP, BACE1 and Aβ production in mouse hippocampus tissue. First, the hippocampus tissue section was analyzed by immunhistochemistry method. The stained yellow positive areas were observed under a light microscope at a magnification of 20 × 100 and 40 × 100 (scale bars, 50 μm and 20 μm, respectively) (A). The Aβ protein expression in the mouse hippocampal zone was evaluated with semiquantitative method (B). Then, the hippocampus tissue section was stained with thioflavin S solution followed by counterstaining nuclei with DAPI. The stained bright green plaques were observed under a fluorescence microscope at a magnification of 20 × 100 and 40 × 100 (scale bars, 50 μm and 20 μm, respectively) (C). The Aβ protein expression in the mouse hippocampal zone was estimated using semiquantitative method (D). Third, the mouse right brain hippocampus tissues were lysed and proteins were extracted; APP and BACE1 protein were detected by western blotting and then analyzed semiquantitatively (E–G). Finally, the RNA of the mouse right brain hippocampus tissue was extracted and then reversely transcribed to cDNA. APP and BACE1 gene expressions were assessed by qRT-PCR and then analyzed semiquantitatively (H, I). Data were represented as mean ± SEM and were analyzed by two-tailed Student’s t-test for comparing the difference between the two groups (n = 3 each group). *P < 0. 05, **P < 0.01, ***P < 0.001