Fig. 4From: Differential paired stage-specific expression of Babesia bovis cysteine-rich GCC2/GCC3 domain family proteins (BboGDP) during development within Rhipicephalus microplusLive immunofluorescence detection of BboGDP1 and BboGDP3 expression on the surface of induced B. bovis extracellular parasites. a B. bovis blood stages and induced sexual stages incubated with anti-BboGDP1 and goat anti-rabbit tagged with AF 647 and stained with Hoechst; the negative control was PI rabbit serum as the primary antibody and stained with Hoechst. b B. bovis blood stages and induced sexual stages incubated with anti-BboGDP3 and goat anti-rabbit tagged with AF 647 and stained with Hoechst; the negative control was PI rabbit serum as the primary antibody and stained with Hoechst. Scale bars: 5 μm. AF 647, Alexa Fluor 647 stain; DAPI, 4,6-diamidino-2-phenylindole dihydrochloride; PI, pre-immuneBack to article page