Wild deer (Pudu puda) from Chile harbor a novel ecotype of Anaplasma phagocytophilum

Parasites & Vectors

Table 1 Primers and thermal conditions used for PCR detection and genetic characterization of Anaplasma and ticks

Organisms	Gene	PCR	Primer	Sequence	T_o (°C)	Expected length (in bp)	Reference
Mammals	GAPDH	Conventional	gapdh F	CCTTCATTGACCTCAACTACAT	52	400	[25]
Mammals	GAPDH	Conventional	gapdh R	CCAAAGTTGTCATGGATGACC	52	400	[25]
Ticks	Mitochondrial 16S rRNA (rrs)	Conventional	16S + 1	CCGGTCTCAACTCAGATCAAGT		460	[26]
Ticks	Mitochondrial 16S rRNA (rrs)	Conventional	16S − 1	GCTCAATGATTTTTTAAATTGCTGT
Anaplasma	16S rRNA (rrs)	Conventional	EC9	TACCTTGTTACGACTT	48	1300	[27]
		Conventional	EC12A	TGATCCTGGCTCAGAACGAACG			[28]
		Nested	A17a	GCGGCAAGCCTCCCACAT	54	1200	[29]
		Nested	IS58-1345r	CACCAGCTTCGAGTTAAACC
	groEl	Conventional	HS1a	AYTGGGCTGGTAYTGAAAT	47	1614	[30]
		Conventional	GroEl_2R	CGTTCTTACTAGGAACATCAAC			[31]
		Nested	Gro677F	ATTACTCAGAGTGCTTCTCARTG	53	942	[32]
		Nested	GroEL_rev2	GCCGACTTTTAGTACAGCAA			[33]
		Heminested	GroEl_2F	TGTAAAGGCGCCTGGTTTCG	55	772	[31]
		Heminested	GroEl_2R	CGTTCTTACTAGGAACATCAAC			[31]
	gltA	Conventional	F4b	CCAGGCTTTATGTCAACTGC	55	800	[34]
		Conventional	R1b	CGATGACCAAAACCCAT
		Nested	EHR-CS136F	TTYATGTCYACTGCTGCKTG	55	650
		Nested	EHR-CS778R	GCNCCMCCATGMGCTGG

GAPDH Glyceraldehyde-3-phosphate dehydrogenase, gltA citrate synthase gene, groEL heat-shock operon

ISSN: 1756-3305