Fig. 4

β-Glucan administration inhibited the hyperactivation of microglia in the PFC of chronic T. gondii Wh6-infected mice. Immunofluorescent staining was carried out to detect the number and morphology of microglia in the PFC of chronic T. gondii Wh6-infected mice. a Representative immunofluorescent staining of the Iba1-positive (Iba1⁺) cells of microglia in the PFC of mice (scale bar: 50 μm). Area in box in each image is enlarged in the respective inset in each image, marked with a solid line (scale bar: 10 μm). Solidity and circularity are used to describe the morphology of microglia. b Quantification of Iba1⁺ microglia in the PFC, c solidity of Iba1⁺ microglia in the PFC, d circularity of Iba1⁺ microglia in the PFC. Values are presented as the mean ± SEM. Asterisks indicate a statistically significant difference at *** P < 0.001, according to one-way ANOVA followed by Tukey’s test for multiple comparisons