Fig. 1

Determination of caspase-1 p20 activated by pcDNA3.1(+)-alpha-2 and alpha-7.3 giardins. The recombinant eukaryotic expression plasmids pcDNA3.1(+)-alpha-2 and alpha-7.3 giardins (above each lane) were transfected into primary mouse peritoneal macrophages, and the medium supernatants were collected at 24 h for measurement of the inflammasome signature protein caspase-1 p20 expression level using western blotting assays. The PBS-only treatment group (lane C) and pcDNA3.1(+)-single treatment group (lane pcDNA3.1) were used as the negative controls, and the GEV treatment group was used as the positive control. The expression of recombinant protein was verified by detecting his-tag in each protein, and the protein bands of the expected size were obtained as alpha-2 giardin (38.2 kDa) and alpha-7.3 giardin (37.2 kDa). GEV, Giardia duodenalis extracellular vesicle; pcDNA3.1(+), EcoRV-linearized vector; SUP, supernatant