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Table 1 Primer sequences of recombinant pcDNA3.1(+)-alpha-2 and alpha-7.3 giardins

From: The NLRP3 inflammasome recognizes alpha-2 and alpha-7.3 giardins and decreases the pathogenicity of Giardia duodenalis in mice

Primer name

Genbank number

Sequence (5ʹ-3ʹ)

Product size (bp)

Alpha-2 giardin

XM_001706906

F:TGGTGGAATTCTGCAGATATGGTTCCGAAGCTATCCCAGA

892

R:GCCGCCACTGTGCTGGATACTCCCTTAGGCGCCAGA

Alpha-7.3 giardin

XM_001708403

F:TGGTGGAATTCTGCAGATATGGCTGCAGCGAAGGCTA

886

R:GCCGCCACTGTGCTGGATACATGACGTGCCAGAGGAC

  1. The underlined parts were introduced bases to enhance the expression efficiency of recombinant pcDNA3.1(+)-alpha-2 and alpha-7.3 giardins
  2. F Forward primer, R reverse primer
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Parasites & Vectors

ISSN: 1756-3305

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