Fig. 2

Effect of increasing temperature on canine IgG antibody interference of antigen detection as measured by DiroCHEK^® via optical density at 650 nm. Pooled heartworm antigen optical density results following mixing of antibody solutions Ab1–4 (heated at 6 temperatures) with four heartworm antigen positive samples Ag1–4 (Graphs A-D) compared to Ag1–4/PBS 25 °C controls. Antigen detection is initially decreased following mixing with antibody solutions and then recovers with increasing temperature relative to PBS control