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Fig. 5 | Parasites & Vectors

Fig. 5

From: Proteomic analysis of extracellular vesicles from tick hemolymph and uptake of extracellular vesicles by salivary glands and ovary cells

Fig. 5

Western blot analysis of EVs purified from Rhipicephalus haemaphysaloides and Hyalomma asiaticum hemolymph. A total of 30 μg of EV protein per lane was loaded. EVs were positive for exosome markers, A RhCD9, B RhTSG101, C GAPDH, D Rh14-3-3ζ, E Rh14-3-3ε and F RhFerritin-2, and were negative for markers of G RhFerritin-1, H RhCalnexin and I RhCytochrome c. J Representative images of immunogold labeling of EVs purified by ultracentrifuge from tick hemolymph. Upper panel, 14–3-3ζ; lower panel, ferritin-2. Red arrows indicate immunogold labeling

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Parasites & Vectors

ISSN: 1756-3305

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